最新产品
NxGen® phi29 DNA Polymerase
NxGen® phi29 DNA Polymerase
货号
30221-1
规格
2,000 U
存储温度
-20º C
产品详情
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Excellent strand displacement, fidelity and speed.
phi29 DNA Polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′→ 5′ proofreading exonuclease function. The enzyme is capable of up to 70,000 insertions per binding event.
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Specifications
Unit Definition: 1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30°C.
Source: A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29.
| Unit Concentration | 10,000 U/mL |
| Purity (SDS-PAGE) | >99% |
| SS Exonuclease | Functional |
| E. coli 16S rDNA Contamination | 100 U <10 copies |
| Endonuclease | 100 U <10% converted |
| Storage | -20&°C |
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Ordering
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Manuals
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Resources
Supplied at 10,000 U/mL in 10 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.5% Tween-20, 0.5% NP-40, 50% glycerol, pH 7.4 @ 25°C. Also supplied is 10X phi29 DNA Polymerase Buffer composed of 500 mM Tris-HCl, 100 mM (NH4)2SO4, 40 mM Dithiothreitol, 100 mM MgCl2, pH 7.5 @ 25°C. 10,000 U is supplied as 5 × 2,000 U.
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