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NxGen® M-MuLV Reverse Transcriptase

NxGen® M-MuLV Reverse Transcriptase

货号

30222-1

规格

50,000 U

存储温度

-20º C

产品详情

NxGen® M-MuLV Reverse Transcriptase

 

产品说明书

 

  • Outstanding cDNA synthesis.

 

NxGen Enzymes Icon M-MuLV Reverse Transcriptase is an RNA-dependent DNA polymerase which shows no measurable 3′→5′ proofreading function. This enzyme can copy a single-stranded DNA template or perform cDNA synthesis by extending a DNA primer annealed to an RNA template.

Specifications

Unit Definition: 1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.

Source: A recombinant E. coli strain carrying the Moloney-Murine Leukemia Virus Reverse Transcriptase gene.

M-MuLV Price Comparison
Unit Concentration 200,000 U/mL
Purity (SDS-PAGE) >99%
SS Exonuclease 2,000 U <5.0% released
DS Exonuclease 2,000 U <0.5% released
Endonuclease 2,000 U <10% converted
E. coli 16S rDNA Contamination 2,000 U <10 copies
Storage -20°C

  • Ordering

  • Manuals

  • Resources

Contact your local distributor for pricing
Product Description Size Cat. No.
NxGen® M-MulV Reverse Transcriptase   50,000 U 30222-1
    250,000 U 30222-2
NxGen® RNAse Inhibitor   10,000 U 30281-1
    50,000 U 30281-2
Pyrophosphatase Inorganic, Thermostable   250 Units 30074-1
    (5 x 250 U) 30074-2
ORDER INFORMATION
Supplied at 200,000 U/mL in 50 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% NP-40 Alternative, 50% glycerol, pH 7.6 @ 25°C. Also provided is 10X M-MuLV RT Buffer which in 1X form is composed of 50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol, pH 8.3 @ 25°C. 250,000 U is supplied as 5 × 50,000 U.
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