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NxGen® M-MuLV Reverse Transcriptase

产品说明书

Outstanding cDNA synthesis.

M-MuLV Reverse Transcriptase is an RNA-dependent DNA polymerase which shows no measurable 3′→5′ proofreading function. This enzyme can copy a single-stranded DNA template or perform cDNA synthesis by extending a DNA primer annealed to an RNA template.

Specifications

Unit Definition: 1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.

Source: A recombinant E. coli strain carrying the Moloney-Murine Leukemia Virus Reverse Transcriptase gene.

Unit Concentration	200,000 U/mL
Purity (SDS-PAGE)	>99%
SS Exonuclease	2,000 U <5.0% released
DS Exonuclease	2,000 U <0.5% released
Endonuclease	2,000 U <10% converted
E. coli 16S rDNA Contamination	2,000 U <10 copies
Storage	-20°C

Ordering
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Product Description	Size	Cat. No.
NxGen® M-MulV Reverse Transcriptase	50,000 U	30222-1
	250,000 U	30222-2
NxGen® RNAse Inhibitor	10,000 U	30281-1
	50,000 U	30281-2
Pyrophosphatase Inorganic, Thermostable	250 Units	30074-1
	(5 x 250 U)	30074-2

ORDER INFORMATION
Supplied at 200,000 U/mL in 50 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% NP-40 Alternative, 50% glycerol, pH 7.6 @ 25°C. Also provided is 10X M-MuLV RT Buffer which in 1X form is composed of 50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol, pH 8.3 @ 25°C. 250,000 U is supplied as 5 × 50,000 U.

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进口试剂

NxGen® M-MuLV Reverse Transcriptase

NxGen® M-MuLV Reverse Transcriptase

Ordering

Manuals

Resources