NxGen® T4 DNA Ligase (High Concentration)

NxGen® T4 DNA Ligase (High Concentration)




1,500 U


-20º C


NxGen® T4 DNA Ligase




  • Reliably join double-stranded nucleic acid chains.


NxGen Enzymes IconT4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

T4 DNA Ligase Version Sizes Ligase Concentration Provided Buffer
Low Concentration Ligase

1,500 U
7,500 U

2 U/µL 10X T4 DNA Ligase Buffer
High Concentration Rapid Kit 1,500 U
7,500 U
10 U/µL 2X Rapid Ligation Buffer,
10X T4 DNA Ligase Buffer

10X T4 DNA Ligase Buffer is composed of 500 mM Tris-HCI, 100 mM MgCl2, 50 mM dithiothreitol, 10 mM ATP, pH 7.6 @ 25 °C.

2X Rapid Ligation Buffer is composed of 132 mM Tris-HCI, 20 mM MgCl2, 2 mM dithiothreitol, 2 mM ATP, 15% PEG, pH 7.6 @ 25 °C.


Figure 1:  Purity
The purity of the T4 DNA ligase is equal or greater than 95% as judged by SDS-polyacrylamide gel electrophoresis with Coomassie blue staining.  Molecular weight markers are in Lane 1.  T4 DNA ligase was loaded at 5 units (Lane 2) and 10 units (Lane 3).


Figure 2:  Exonuclease and Endonuclease Activity Assay
The T4 DNA ligase is free of detectable of exo- and endonuclease activities, as judged by agarose gel electrophoresis following incubation of 10 units of enzyme for 16 hours at 37 °C with 1 µg of HindIII-digested λ DNA (Lanes 3 and 4) and supercoiled pUC19 DNA (Lanes 5 and 6).  Molecular weight markers are in Lanes 1 and 2.


Unit Definition: One Weiss Unit is defined as the amount of enzyme required to convert 1 nmol of 32P-labeled inorganic pyrophosphate into Norit adsorbable material in 20 minutes at 37 °C, using specified reaction conditions. Note: 1 Weiss Unit is approximately 67 cohesive end units.

Source: A recombinant E. coli strain carrying the cloned T4 DNA Ligase gene.

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Product Description Size Cat. No.
NxGen® T4 DNA Ligase (Low Concentration)   1,500 U 30241-1
    7,500 U 30241-2
NxGen® T4 DNA Ligase (High Concentration)   1,500 U 30243-1
    7,500 U 30243-2

Storage buffer: T4 DNA Ligase is supplied in 10 mM Tris-HCl, 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 0.1%Triton X-100, 50% glycerol, pH 7.5 @ 25 °C.