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CloneDirect™ Rapid Ligation Kit

CloneDirect™ Rapid Ligation Kit

货号

40020-5

规格

96 rxns

存储温度

-20º C

产品详情

CloneDirect™ Rapid Ligation Kit

 

产品说明书

 

  • Ligation reaction is transformation-ready in minutes
  • No DNA cleanup step required
  • Highly reliable and reproducible results
 

Quicken cloning

The CloneDirect Rapid Ligation Kit is a simple, rapid, highly efficient alternative to standard ligation and transformation protocols. This kit eliminates the requirement for a post-ligation cleanup step before electroporation. A 15-minute heat denaturation at 70°C is all that is needed prior to cell transformation.

Gel showing CloneDirect

Figure 1. Ligation efficiencies measured by transformation. Ligation reactions were heat denatured at 70°C and used without additional cleanup to transform electrocompetent E. cloni® 10G cells. Transformants (in millions) are shown.

  • Ordering

  • Manuals

  • Resources

Contact your local distributor for pricing
Product Description Size Cat. No.
CloneDirect Rapid Ligation Kit   24 rxns 40020-2
    48 rxns 40020-4
    96 rxns 40020-5
ORDER INFORMATION
The CloneDirect Rapid Ligation Kit contains: 10X CloneDirect Buffer (contains ATP), CloneSmart® DNA Ligase, and complete protocols.
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