DNATerminator® End Repair Kit

DNATerminator® End Repair Kit


货号

40035-2


规格

50 rxns


存储温度

-20º C


产品详情


DNATerminator® End Repair Kit

 

产品说明书

 

  • Repair sheared or restriction-digested DNA ends
  • Increase cloning efficiency as much as a 500%
  • Optimized system for consistent & reliable results
 

Repair DNA ends

Double-stranded DNA fragments for library construction or large-scale sequencing are often generated by mechanically shearing larger (genomic) DNA, a process that primarily leaves uneven ends. Large DNA may also be cut with restriction enzymes to generate smaller fragments for subcloning. Sheared or restricted DNA fragments must be “end-repaired” before ligation into blunt-end cloning vectors (e.g., Lucigen’s CloneSmart®, CopyRight®, or BigEasy® systems).

The DNATerminator End Repair Kit creates blunt, 5' phosphorylated ends on any type of sheared or restriction-digested DNA fragment, ensuring the highest possible cloning efficiency (Figure 1).

DNA Terminator Kit

Figure 1. Effect of DNA shearing and end-repair methods on library construction efficiency. Shotgun libraries were constructed using 250 ng of lambda DNA sheared by sonication (Son) or by the GeneMachines® HydroShear™ device (HS). Sheared DNA was repaired with T4 DNA polymerase and Klenow fragment (TK) or DNATerminator End Repair Kit (DT) and cloned using Lucigen’s CloneSmart® system. Values are colony forming units (cfu) per library.

  • Ordering

  • Manuals

  • Resources

Contact your local distributor for pricing
Product Description Size Cat. No.
DNATerminator® End Repair Kit   10 rxns 40035-1
    50 rxns 40035-2
    20 rxns 40035-3
ORDER INFORMATION
The DNATerminator End Repair Kit contains: DNATerminator End Repair Enzyme Mix, End Repair Buffer, and a complete protocol.