CopyRight® v2.0 BAC Cloning Kit, pSMART® BAC BamHI with BAC-Optimized Replicator™ v2.0 Electrocompetent Cells

CopyRight® v2.0 BAC Cloning Kit, pSMART® BAC BamHI with BAC-Optimized Replicator™ v2.0 Electrocompetent Cells


货号

42030-1


规格

10 rxns


存储温度

-20º & -80º C


产品详情


CopyRight® v2.0 BAC Cloning Kits (pSMART® BAC and pEZ™ BAC)

 

产品说明书

 

  • 有效克隆任何大小在200kb的片段
  • 从A/T或G/C丰富的基因组创建文库
  • Clone gene clusters or operons.
  • 高插入稳定性
  • 诱导拷贝数

Lucigen's CopyRight® v2.0 BAC克隆试剂盒带有 pSMART® BAC (Figure1)或 pEZ BAC (Figure 2) 载体和适用于BAC文库优化的感受态细胞,确保文库的准确性,可靠性和有效性。这些载体整合了CloneSmart®无转录技术,最小化克隆的偏好性,有利于克隆产毒素基因。随着诱导拷贝数的扩增(20到50倍)可获得更高的DNA量。LacZ-sacB片段可以消除未切割载体所形成的假阳性克隆。不像其他BAC克隆系统,Lucigen's CloneSmart技术确保了未克隆化的插入也能稳定遗传。在克隆过程中,序列不容易发生丢失或重排。CopyRigh盒子与其他同类盒子相比,以较好的价格保证了最优的BAC克隆。图3显示了一个用pSMART BAC 载体构建的随机剪切的BAC文库。

pSMART BAC Vector Map

Figure 1. CopyRight vector pSMART BAC.  ori2, repE, IncC - single-copy origin of replication; oriV - inducible origin of replication; par A,B,C - partition genes; Cm^r - chloramphenicol-resistance gene; cosN - lambda packaging signal; T - CloneSmart transcription terminators; sacB -sucrase gene. Approximate positions of sequencing primers and transcription terminators are indicated.

pEZ BAC Vector Map

Figure 2. CopyRight vector pEZ BAC. ori2, repE, IncC, single-copy origin of replication; oriV - inducible origin of replication; par A,B,C, partition genes; Cm^r - chloramphenicol-resistance gene; cosN - lambda packaging signal; lacZ - alpha peptide of the beta galactosidase gene. Approximate positions of sequencing primers and transcription terminators are indicated.

Arabidopsis BAC gel

Figure 3. Arabidopsis Random Shear BAC Library in the pSMART BAC vector. 7,680 clones were obtained, with an average insert size of 105 kb and ~5X genome coverage.

易于成功
有效的获得克隆或创建文库。the CopyRight v2.0克隆盒子消除了繁琐的载体和感受态细胞制备过程,也省去了QC试验的耗时。盒子包括最优化的试剂,连接用载体,高效感受态细胞,详细说明书和疑惑解答。

  • Ordering

  • Manuals

  • Resources

Contact your local distributor for pricing
Product Description Size Cat. No.
BAC-Optimized Replicator v2.0 Electrocompetent Cells   12 rxns (DUOs) 60210-1
    24 rxns (DUOs) 60210-2
CopyRight v2.0 BAC Cloning Kit, pSMART BAC BamHI w/BAC-Optimized Replicator v2.0 Electrocompetent Cells   10 rxns 42030-1
    20 rxns 42030-2
CopyRight v2.0 BAC Cloning Kit, pSMART BAC HindIII w/BAC-Optimized Replicator v2.0 Electrocompetent Cells   10 rxns 42032-1
    20 rxns 42032-2
CopyRight v2.0 pEZ BAC Blunt Cloning Kit w/Electrocompetent Cells   10 rxns 42009-1
    20 rxns 42009-2
CopyRight v2.0 pEZ BAC Blunt Cloning Kit without cells   10 rxns 42016-1
ORDER INFORMATION

每一个CopyRight® v2.0 BAC克隆盒子都包括:切割载体(pSMART BAC带有BamHI 或HindIII位点),CopyRight DNA连接酶,CopyRight 5X连接buffer,阳性对照插入DNA,测序引物,BAC优化的Replicator v2.0电转感受态细胞(一管可做两次转化),复苏培养基,阿拉伯糖诱导溶液和操作手册。
每一个CopyRight pEZ BAC克隆盒子含有: 切割CopyRight载体(pEZ BAC 平端)CloneSmart DNA连接酶,CloneDirect™10 X连接buffer(包括ATP),阳性对照插入DNA,拷贝诱导溶液,测序引物和操作手册。盒子所带细胞还包括诱导BAC最适的Replicator v2.0感受态细胞(>1 × 1010 cfu/µg DNA),每管可做两次转化,阳性对照质粒和复苏培养基。

Please note that the following vectors have been discontinued: pSMART BAC EcoRI (catalog numbers 42031-1, 42031-2) and pEZ BAC BamHI (catalog numbers 42007-1, 42007-2 and 42012-1).”