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Expresso® Solubility and Expression Screening System
Expresso® Solubility and Expression Screening System
货号
49060-1
规格
24 rxns
存储温度
-20º C
产品详情
Multiplex your protein solubility screen
这是一个方便的筛选盒子，含有七个融合性标签可以增加蛋白表达和可溶性。
- 使用一个扩增子获得您所构建的全部产物
- 通过TEV切割融合标签进行快速，同步的蛋白筛选
- 从PCR到蛋白获得不到4天时间。
- Overview
- Simplified workflow
- Results
- Fusion tags included
- Protein cleavage with SelecTEV™ Protease
- FAQ's
概述
The Expresso® 可溶性表达系统是一个完整的系统可以完成PCR扩增，快速克隆以及表达您所感兴趣的蛋白，通过一个独特的标签切割系统来实现。Lucigen已经鉴定了7个独特有效的可溶性标签，并且将他们与表达技术结合创立了一套克隆系统用于快速同时评价你的蛋白带有7个可溶标签（加上一个6组氨酸标签的对照载体）。
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Figure 1. Each pSOL vector contains everything you need to seamlessly clone in your target gene without any additional amino acids added. Fusion tags are cleavable via a TEV protease site. |
Simplified Workflow
Lucigen的表达技术使这项工作比以往更简单快速，可以快速筛选多融合标签，提高了蛋白的表达和可溶性。使用一个扩增子同时产生8个不同克隆（7个融合标签的和一个带组氨酸标签的对照），接下来筛选表达的可溶性蛋白
结果
每个蛋白对于不同的融合标签反应不同。使用可溶性表达筛选系统，你可以快速，容易的大量筛选，筛选到对于你的蛋白最适的融合标签。
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| Figure 2. GH1 (Human Growth Hormone) was evaluated for E. coli expression and solubility when expressed as a fusion. Fusion tags SlyD, Tsf, SUMO, Bla, and MBP demonstrated enhanced solubility (Lane S) compared to the His-control protein. T: Total Lysate, S: Cleared Soluble Fraction, I: Insoluble Pellet |
Fusion Tags Included
The Expresso Solubility and Expression Screening System includes 7 vectors that each contain a unique fusion tag and one His control vector. The fusion tags vary in sequence, size, and derivative. Below is a table summarizing the characteristics of each fusion tag:
|
# |
Fusion Tag |
AA Length |
kDa* |
pI |
Description |
|
1 |
6xHis-AFV |
113 |
13.5 |
5.0 |
Hypothetical protein from Acidianus filamentous virus 1 |
|
2 |
6xHis-SlyD |
210 |
22.7 |
5.2 |
FKBP-type peptidyl-prolyl cis-trans isomerase |
|
3 |
6xHis-Tsf |
297 |
32.2 |
5.7 |
E. coli elongation factor |
|
4 |
6xHis-SUMO |
115 |
13.3 |
5.2 |
Small Ubiquitin-like Modifier |
|
5 |
6xHis-Bla |
381 |
41.3 |
4.4 |
Beta-lactamase |
|
6 |
6xHis-MBP |
382 |
42.1 |
5.5 |
Maltose-Binding Protein |
|
7 |
6xHis-GST |
233 |
27.4 |
6.6 |
Glutathione S-transferase |
|
8 |
6xHis Control |
14 |
1.8 |
7.0 |
Affinity tag |
Cleavage with SelecTEV® Protease
All fusion tagged proteins produced with the Expresso Solubility and Expression Screening System are fully cleavable with SelecTEV protease from Lucigen. For more information, visit the SelecTEV protease product page
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| Figure 3. Coomassie-stained SDS-PAGE gel of TEV cleavage reaction. 10 U of SelecTEV Protease was incubated with 30 µg of fusion protein substrate at 30°C for 1 hour in a 100 µL reaction. Marker (lane M), SelecTEV Protease (Lane 1), Uncleaved fusion substrate (Lane 2), Cleaved substrate and SelecTEV Protease. (Lane 3) |
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Ordering
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Manuals
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Resources
包含的融合蛋白
可溶性表达筛选系统包括 7个载体，每一个载体含有一个独特的可溶标签和一个组氨酸对照载体。融合标签在序列，大小和来源上都不同。下面的表格总结了每种融合标签的特点。
Contact your local distributor for pricing
The Expresso Solubility and Expression Screening System contains pre-processed pSol™ vectors with 7 different fusion tags and one HIS-tagged control vector (3 reactions of each vector), positive control insert, sequencing primers, E. cloni® 10G Chemically Competent Cells (SOLOs), and 20% Rhamnose solution and 15% Glucose solution autoinduction reagents. SelecTEV™ Protease and Accura® High-Fidelity Polymerase are available for purchase separately.
For research use only. Complete protocols are available online at www.lucigen.com/manuals.
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