E. cloni® 10G 96-well Chemically Competent Cells

E. cloni® 10G 96-well Chemically Competent Cells


货号

60096-4


规格

4 plates


存储温度

-80º C


产品详情


E. cloni® 10G and 10GF' Chemically Competent Cells

产品说明书

  • Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)
  • Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable.
  • Available in a range of transformation efficiencies (1 × 106 to 1 x 109cfu/µg).
  • Convenient packaging options.
  • High efficiency cells result in high colony counts
  • Endorsed by SGI-DNA for Gibson Assembly® applications

Looking for electrocompetent version?

Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols.  E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required.  These cells provide solutions for a wide range of applications at economical prices.

Choice of strain:

  • E. cloni 10G Competent Cells. Routine cloning, subcloning, and plasmid isolation with or without blue/white screening.
  • E. cloni 10GF′ Competent Cells. Contain the F′ plasmid for infection with M13 to produce ssDNA

Choice of efficiency:

  • E. cloni 10G Chemically Competent Cells (> 1 × 109 cfu/µg pUC DNA; (> 1 × 108 in 96-well plates). 
    Highly efficient competent cells for routine cloning applications.  Available in one transformation per tube (SOLOs) or two transformations per tube (DUOs).  Also available in 96-well plates (which can be divided into 24-well seqments) for higher throughput.
  • E. cloni 10GF´ Chemically Competent Cells (> 5 × 108 cfu/µg pUC DNA).  
    An excellent choice for producing single-stranded DNA for sequencing, mutagenesis, etc.
  • E. cloni 10G Chemically Competent Cells, Subcloning Grade (> 1 × 106 cfu/µg pUC DNA).  
    The best value available anywhere for simple cloning and plasmid propagation.

 

Custom CellsCustom Competent Cells

Bulk Lucigen cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing! See Custom Competent Cells page for detailed information.


Table 1. E. cloni Competent Cells for Cloning


E. cloni Cell Lines

Transformation Efficiency
(cfu/µg pUC DNA)

Cloning Methylated DNA

BAC, Cosmid Cloning

Blue/White Screening

10G Chemically Competent

>1 × 109

YES

NO

YES: without IPTG induction

10G Chemically Competent Subcloning Grade

>1 × 106

YES

NO

YES: without IPTG induction

10GF´ Chemically Competent

>5 × 108

YES

NO

YES: IPTG induction required

Genotypes

  • E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galgalrpsnupG λ- tonA
  • E. cloni 10GF′: [F´ pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galgalrpsnupGλ tonA

Efficiencies

  • E. cloni 10G Chemically Competent Cells  > 1 × 109 cfu/µg pUC DNA;
      96-well format > 1 × 108 in 96-well plates
  • E. cloni 10GF´ Chemically Competent Cells > 5 × 108 cfu/µg pUC DNA
 
  • Ordering

  • Specifications

  • Manuals

  • Resources

Contact your local distributor for pricing
Filter products by:
Size:
 
[i] Transformations/Tube:
 
Efficiency:
 
 
Product Description Size Cat. No.
E. cloni 10G Chemically Competent Cells > 1 × 10^9 cfu/µg   4 rxns 60107-0
    12 rxns (DUOs) 60107-1
    24 rxns (DUOs) 60107-2
    48 rxns (DUOs) 60107-3
    96 rxns (DUOs) 60107-4
E. cloni 10G Chemically Competent Cells > 1 × 10^9 cfu/µg   12 rxns (SOLOs) 60106-1
    24 rxns (SOLOs) 60106-2
    48 rxns (SOLOs) 60106-3
E. cloni 10G Chemically Competent Cells (Subcloning Grade) > 1 × 10^6 cfu/µg   48 rxns 60108-1
    96 rxns 60108-2
E. cloni 10G Chemically Competent Cells > 1 × 10^8 in 96-well plates   1 plate (SOLOs) 60096-1
    4 plates (SOLOs) 60096-4
E. cloni 10GF' Chemically Competent Cells   24 rxns (DUOs) 60062-2
E. cloni 10GF' Chemically Competent Cells > 5 × 10^8 cfu/µg   12 rxns (DUOs) 60062-1
ORDER INFORMATION

Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), Subcloning Grade (12 transformations per tube), or microplates as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

PLEASE NOTE: Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all Lucigen Competent Cells. Please contact Lucigen.