E. cloni® 10G Chemically Competent Cells

E. cloni® 10G Chemically Competent Cells




12 rxns (SOLOs)


-80º C



E. cloni® 10G Chemically Competent Cells






·         可直接取代克隆标准菌株 (DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)

·         优化遗传背景适用于高产量表达:噬菌体T1抗性,内切酶和重组缺陷,蓝白斑筛选。

·         有效的转化效率(1 × 106  1 x 109cfu/µg)

·         便捷的包装选择

·         高效的克隆数

·          Gibson Assembly® applications 签署研发

Lucigen的 E. cloni感受态细胞享有标准克隆菌株(如DH5α™, DH10B™, JM109, TOP10等)优化的遗传元件,并且直接取代了这些菌株的克隆方法。E. cloni化学感受态细胞非常方便研究人员使用-只需要简单的实验室相关设备。这使得该细胞得到了广泛的应用,而且价格实惠。

E. cloni 10G 感受态细胞传统克隆,亚克隆和利用或不利用蓝白斑筛选的质粒提取。


o    E. cloni 10G 化学感受态细胞 (> 1 × 109 cfu/µg pUC DNA; (> 1 × 108 in 96空培养板).

o    E. cloni 10G 化学感受态细胞亚克隆级 (> 1 × 106 cfu/µg pUC DNA).  

Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols.  E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required.  These cells provide solutions for a wide range of applications at economical prices.

Choice of strain:

  • E. cloni 10G Competent Cells. Routine cloning, subcloning, and plasmid isolation with or without blue/white screening.

  • E. cloni 10GF′ Competent Cells. Contain the F′ plasmid for infection with M13 to produce ssDNA

Choice of efficiency:

  • E. cloni 10G Chemically Competent Cells (> 1 × 109 cfu/µg pUC DNA; (> 1 × 108 in 96-well plates). 
    Highly efficient competent cells for routine cloning applications.  Available in one transformation per tube (SOLOs) or two transformations per tube (DUOs).  Also available in 96-well plates (which can be divided into 24-well seqments) for higher throughput.
  • E. cloni 10GF´ Chemically Competent Cells (> 5 × 108 cfu/µg pUC DNA).  
    An excellent choice for producing single-stranded DNA for sequencing, mutagenesis, etc.
  • E. cloni 10G Chemically Competent Cells, Subcloning Grade (> 1 × 106 cfu/µg pUC DNA).  
    The best value available anywhere for simple cloning and plasmid propagation.


Custom CellsCustom Competent Cells

Bulk Lucigen cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing! See Custom Competent Cells page for detailed information.

Table 1. E. cloni Competent Cells for Cloning

E. cloni Cell Lines

Transformation Efficiency
(cfu/µg pUC DNA)

Cloning Methylated DNA

BAC, Cosmid Cloning

Blue/White Screening

10G Chemically Competent

>1 × 109



YES: without IPTG induction

10G Chemically Competent Subcloning Grade

>1 × 106



YES: without IPTG induction

10GF´ Chemically Competent

>5 × 108



YES: IPTG induction required


  • E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galgalrpsnupG λ- tonA
  • E. cloni 10GF′: [F´ pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galgalrpsnupGλ tonA


  • E. cloni 10G Chemically Competent Cells  > 1 × 109 cfu/µg pUC DNA;
      96-well format > 1 × 108 in 96-well plates
  • E. cloni 10GF´ Chemically Competent Cells > 5 × 108 cfu/µg pUC DNA
产品名称 规格  货号
E. cloni 10G Chemically Competent Cells > 1 × 10^9 cfu/µg   4 rxns 60107-0
    12 rxns (DUOs) 60107-1
    24 rxns (DUOs) 60107-2
    48 rxns (DUOs) 60107-3
    96 rxns (DUOs) 60107-4
E. cloni 10G Chemically Competent Cells > 1 × 10^9 cfu/µg   12 rxns (SOLOs) 60106-1
    24 rxns (SOLOs) 60106-2
    48 rxns (SOLOs) 60106-3
E. cloni 10G Chemically Competent Cells (Subcloning Grade) > 1 × 10^6 cfu/µg   48 rxns 60108-1
    96 rxns 60108-2
E. cloni 10G Chemically Competent Cells > 1 × 10^8 in 96-well plates   1 plate (SOLOs) 60096-1
    4 plates (SOLOs) 60096-4
E. cloni 10GF' Chemically Competent Cells   24 rxns (DUOs) 60062-2
E. cloni 10GF' Chemically Competent Cells > 5 × 10^8 cfu/µg   12 rxns (DUOs) 60062-1

Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), Subcloning Grade (12 transformations per tube), or microplates as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

PLEASE NOTE: Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all Lucigen Competent Cells. Please contact Lucigen.