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E. cloni® 10G Chemically Competent Cells
E. cloni® 10G Chemically Competent Cells
货号
60106-2
规格
24 rxns (SOLOs)
存储温度
-80º C
产品详情
E. cloni® 10G Chemically Competent Cells
· 可直接取代克隆标准菌株 (如DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)
· 优化遗传背景适用于高产量表达：噬菌体T1抗性，内切酶和重组缺陷，蓝白斑筛选。
· 有效的转化效率(1 × 106 到 1 x 109cfu/µg)
· 便捷的包装选择
· 高效的克隆数
· 由 Gibson Assembly® applications 签署研发
Lucigen 的 E. cloni 感受态细胞享有标准克隆菌株（如DH5α™, DH10B™, JM109, TOP10等）优化的遗传元件，并且直接取代了这些菌株的克隆方法。E. cloni化学感受态细胞非常方便研究人员使用-只需要简单的实验室相关设备。这使得该细胞得到了广泛的应用，而且价格实惠。
E. cloni 10G 感受态细胞. 传统克隆，亚克隆和利用或不利用蓝白斑筛选的质粒提取。
效率选择：
o E. cloni 10G 化学感受态细胞 (> 1 × 109 cfu/µg pUC DNA; (> 1 × 108 in 96空培养板).
高效率的感受态细胞用于常规克隆。没管可做一次转化或每管做两次转化。也可用于96孔板（可用于24孔），用于高通量转化。
o E. cloni 10G 化学感受态细胞, 亚克隆级 (> 1 × 106 cfu/µg pUC DNA).
主要用于简单的克隆或是质粒扩增。
Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required. These cells provide solutions for a wide range of applications at economical prices.
Choice of strain:
-
E. cloni 10G Competent Cells. Routine cloning, subcloning, and plasmid isolation with or without blue/white screening.
- E. cloni 10GF′ Competent Cells. Contain the F′ plasmid for infection with M13 to produce ssDNA
Choice of efficiency:
-
E. cloni 10G Chemically Competent Cells (> 1 × 109 cfu/µg pUC DNA; (> 1 × 108 in 96-well plates).
Highly efficient competent cells for routine cloning applications. Available in one transformation per tube (SOLOs) or two transformations per tube (DUOs). Also available in 96-well plates (which can be divided into 24-well seqments) for higher throughput. -
E. cloni 10GF´ Chemically Competent Cells (> 5 × 108 cfu/µg pUC DNA).
An excellent choice for producing single-stranded DNA for sequencing, mutagenesis, etc. -
E. cloni 10G Chemically Competent Cells, Subcloning Grade (> 1 × 106 cfu/µg pUC DNA).
The best value available anywhere for simple cloning and plasmid propagation.
Custom Competent Cells
Bulk Lucigen cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing! See Custom Competent Cells page for detailed information.
Table 1. E. cloni Competent Cells for Cloning
|
E. cloni Cell Lines |
Transformation Efficiency |
Cloning Methylated DNA |
BAC, Cosmid Cloning |
Blue/White Screening |
|
10G Chemically Competent |
>1 × 109 |
YES |
NO |
YES: without IPTG induction |
|
10G Chemically Competent Subcloning Grade |
>1 × 106 |
YES |
NO |
YES: without IPTG induction |
|
10GF´ Chemically Competent |
>5 × 108 |
YES |
NO |
YES: IPTG induction required |
Genotypes
- E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galU galK rpsL nupG λ- tonA
- E. cloni 10GF′: [F´ pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupGλ tonA
Efficiencies
-
E. cloni 10G Chemically Competent Cells > 1 × 109 cfu/µg pUC DNA;
96-well format > 1 × 108 in 96-well plates - E. cloni 10GF´ Chemically Competent Cells > 5 × 108 cfu/µg pUC DNA
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