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E. cloni® 10G Chemically Competent Cells
E. cloni® 10G Chemically Competent Cells
货号
60107-3
规格
48 rxns (DUOs)
存储温度
-80º C
产品详情
E. cloni® 10G and 10GF' Chemically Competent Cells
- Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)
- Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable.
- Available in a range of transformation efficiencies (1 × 106 to 1 x 109cfu/µg).
- Convenient packaging options.
- High efficiency cells result in high colony counts
- Endorsed by SGI-DNA for Gibson Assembly® applications
Looking for electrocompetent version?
Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required. These cells provide solutions for a wide range of applications at economical prices.
Choice of strain:
- E. cloni 10G Competent Cells. Routine cloning, subcloning, and plasmid isolation with or without blue/white screening.
- E. cloni 10GF′ Competent Cells. Contain the F′ plasmid for infection with M13 to produce ssDNA
Choice of efficiency:
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E. cloni 10G Chemically Competent Cells (> 1 × 109 cfu/µg pUC DNA; (> 1 × 108 in 96-well plates).
Highly efficient competent cells for routine cloning applications. Available in one transformation per tube (SOLOs) or two transformations per tube (DUOs). Also available in 96-well plates (which can be divided into 24-well seqments) for higher throughput. -
E. cloni 10GF´ Chemically Competent Cells (> 5 × 108 cfu/µg pUC DNA).
An excellent choice for producing single-stranded DNA for sequencing, mutagenesis, etc. -
E. cloni 10G Chemically Competent Cells, Subcloning Grade (> 1 × 106 cfu/µg pUC DNA).
The best value available anywhere for simple cloning and plasmid propagation.
Custom Competent Cells
Bulk Lucigen cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing! See Custom Competent Cells page for detailed information.
Table 1. E. cloni Competent Cells for Cloning
|
E. cloni Cell Lines |
Transformation Efficiency |
Cloning Methylated DNA |
BAC, Cosmid Cloning |
Blue/White Screening |
|
10G Chemically Competent |
>1 × 109 |
YES |
NO |
YES: without IPTG induction |
|
10G Chemically Competent Subcloning Grade |
>1 × 106 |
YES |
NO |
YES: without IPTG induction |
|
10GF´ Chemically Competent |
>5 × 108 |
YES |
NO |
YES: IPTG induction required |
Genotypes
- E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galU galK rpsL nupG λ- tonA
- E. cloni 10GF′: [F´ pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupGλ tonA
Efficiencies
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E. cloni 10G Chemically Competent Cells > 1 × 109 cfu/µg pUC DNA;
96-well format > 1 × 108 in 96-well plates - E. cloni 10GF´ Chemically Competent Cells > 5 × 108 cfu/µg pUC DNA
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Ordering
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Specifications
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Manuals
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Resources
Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), Subcloning Grade (12 transformations per tube), or microplates as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
PLEASE NOTE: Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all Lucigen Competent Cells. Please contact Lucigen.
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