BAC-Optimized Replicator™ v2.0 Electrocompetent Cells

BAC-Optimized Replicator™ v2.0 Electrocompetent Cells




12 rxns (DUOs)


-80º C


AC-Optimized Replicator™ v2.0 & 10G BAC-Optimized Electrocompetent Cells




  • Cells that enable the most challenging library construction and screening.
  • Highest available transformation efficiencies for library construction and screening.
  • Optimized for high yields of DNA.

Lucigen offers two bacterial strains designed for BAC library construction or transformation of large constructs:

BAC-Optimized Replicator™ v2.0 Electrocompetent Cells (> 1 × 1010 cfu/µg pKanR DNA).  These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields.  BAC-Optimized Replicator Cells are components of Lucigen’s CopyRight®Cloning Kits.

E. cloni 10G BAC-Optimized Electrocompetent Cells (> 1 × 107 cfu/µg 150 kb BAC DNA; > 1 × 1010 cfu/µg pUC DNA). The first competent cells developed by Lucigen specifically for large DNA cloning.  Manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts, 10G BAC-Optimized Cells are an excellent choice for BAC library construction and large construct transformation.

Table 1. E. cloni Competent Cells for BAC or Large Fragment Cloning

E. cloni Cell Lines

Transformation Efficiency
(cfu/µg pUC or pKanR DNA)

Cloning Methylated DNA

BAC, Cosmid Cloning

Blue/White Screening

10G BAC-Optimized Electrocompetent

>1 × 1010



without IPTG induction

BAC-Optimized Replicator v2.0 Electrocompetent

>1 × 1010



IPTG induction required

  • Ordering

  • Specifications

  • Manuals

  • Resources

Contact your local distributor for pricing
Product Description Size Cat. No. Price Quantity BUY
BAC-Optimized Replicator v2.0 Electrocompetent Cells   12 rxns (DUOs) 60210-1 --  
    24 rxns (DUOs) 60210-2 --  
Recovery Medium   8 × 12 ml 80026-1 --  
E. cloni 10G BAC-Optimized Electrocompetent Cells   10 rxns (Fivers) 60215-1 --  
    25 rxns (Fivers) 60215-2 --  
Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube) or FIVERs (5 transformations per tube), as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

PLEASE NOTE: Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all Lucigen Competent Cells. Please contact Lucigen.