E. cloni® 5-alpha Chemically Competent Cells

E. cloni® 5-alpha Chemically Competent Cells




12 rxns (DUOs)


-80º C


E.cloni® 5-alpha Chemically Competent Cells




  • Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.).
  • Optimized genetics for high yields: endonuclease and recombination minus, for blue/white screening-capable.

Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™ and directly replace them in cloning protocols.  E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required.  These cells provide solutions for a wide range of applications at economical prices.

E. cloni 5-alpha Chemically Competent Cells >1 × 108 cfu/µg DNA
Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening.

Red arrows Custom Competent Cells

Bulk Lucigen cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing! See Custom Competent Cells page for detailed information.

Table 1. E. cloni Competent Cells for Cloning

E. cloni Cell Lines

Transformation Efficiency
(cfu/µg pUC DNA)

Cloning Methylated DNA

BAC, Cosmid Cloning

Blue/White Screening

5-alpha Chemically Competent

>1 × 108 cfu/µg DNA



No IPTG induction required


  • Ordering

  • Specifications

  • Manuals

  • Resources

Contact your local distributor for pricing
Product Description Size Cat. No.
E. cloni 5-alpha Chemically Competent Cells >1 × 10^8 cfu/µg DNA   12 rxns (DUOs) 60602-1
    24 rxns (DUOs) 60602-2

Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

PLEASE NOTE: Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all Lucigen Competent Cells.